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Table 1 Nucleotide differences between the reference NDV and the assembled full-length clone, pFLC-LS1

From: Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein

Nucleotide Positiona Nucleotide change Gene Observationc
Reference Full-length clone
4291 A G M Creation of BssHII
4552 CA GC F Creation of NruI
6294 CG GC F-HNb Creation of MluI
8352 CAGCTC TACGTA HN-Lb Creation of SnaBI
8817 A G L per se mutation
9440 T C L per se mutation
11,657 G A L per se mutation
12,567 C T L per se mutation
14,608 T A L Destruction of BbvCI
  1. a Numbering of the nucleotide position is relative to the reference NDV (GenBank sequence no. Y18898)
  2. bIntergenic regions between F and HN or between HN and L genes, respectively
  3. cDifferences in the cDNA sequence were caused by introduction/destruction of artificial restriction sites (in fragments-1 and -2) or per se the genome sequence (in fragment-3)