Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein

Table 1 Nucleotide differences between the reference NDV and the assembled full-length clone, pFLC-LS1

Nucleotide Position^a	Nucleotide change		Gene	Observation^c
Nucleotide Position^a	Reference	Full-length clone	Gene	Observation^c
4291	A	G	M	Creation of BssHII
4552	CA	GC	F	Creation of NruI
6294	CG	GC	F-HN^b	Creation of MluI
8352	CAGCTC	TACGTA	HN-L^b	Creation of SnaBI
8817	A	G	L	per se mutation
9440	T	C	L	per se mutation
11,657	G	A	L	per se mutation
12,567	C	T	L	per se mutation
14,608	T	A	L	Destruction of BbvCI

^a Numbering of the nucleotide position is relative to the reference NDV (GenBank sequence no. Y18898)
^bIntergenic regions between F and HN or between HN and L genes, respectively
^cDifferences in the cDNA sequence were caused by introduction/destruction of artificial restriction sites (in fragments-1 and -2) or per se the genome sequence (in fragment-3)

ISSN: 1743-422X