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Fig. 3 | Virology Journal

Fig. 3

From: Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein

Fig. 3

Identification of the rescued rLS1 virus. a DF-1 cells infected with rLS1 at an MOI of 0.01. After 48 h, the cells were fixed and the presence of NDV was detected using a NDV-specific monoclonal antibody against ribonucleoprotein. Infected cells showed cell fusion and formation of syncytia (yellow arrow) ×200. b Confirmation of the recovered rLS1 virus by RT-PCR and restriction digestion. Genomic RNA isolated from recovered rLS1 and LaSota viruses was amplified by RT-PCR with specific primers covering the created (lanes 1 and 3) or destroyed restriction sites (lanes 2 and 4), and the products analyzed on a 2% agarose gel. Lane M: 50 bp molecular size marker; lane 1: BssHII for rLS1 (192 bp and 103 bp); lane 2: BbvCI for rLS1 (969 bp); lane 3: BssHII for LaSota (295 bp); lane 4: BssHII for LaSota (573 bp and 396 bp)

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