Table 1 PCR primers of RdRp, S genes of four HCoVs
From: Prevalence and genetic diversity analysis of human coronaviruses among cross-border children
Target genes | HCoVs | Primer | Sequence (5′- 3′) | Location |
|---|---|---|---|---|
RdRp | OC43 | F | CGAGTGTAGATGCCCGTCTCG | 13,353-13,373 |
R | GCATCTGTCTTAACAACATCATC | 15,990-15,970 | ||
HKU1 | F | GAATGCCCGGCTAGTACCCTGTGC | 13,581-13,604 | |
R | GGGTAAGCATCTATAGCTAGAC | 16,127-16,106 | ||
NL63 | F | GGCACGGACATCGATAAGTGTG | 112,481-12,505 | |
R | GCATCTGTCTTAACAACATCATC | 14,954-14,932 | ||
229E | F | CTGAAGTCCAATTGTGTGCGC | 12,493-12,513 | |
R | CACCTTCGTTAAGAGTCTTGTTGAG | 15,034-15,010 | ||
S | OC43 | F | TCCCTGATTTACCCATTTGTG | 23,486-23,506 |
R | ATAGTTAATGGGTTGCAGCTGT | 25,807-25,786 | ||
HKU1 | F-1 | TATGTTAATAAWACTTTGTATAGTG | 23,236-23,260 | |
R-1 | TACAATTGACAAGAACTAGAAG | 24,179-24,158 | ||
F-2 | ACCTCTTAATTGGGAACGTA | 23,922-23,941 | ||
R-2 | GAAGATCTCTAATTTCACTACCAC | 25,717-25,694 | ||
NL63 | F | GAGTGGTAGGTTGTTGTTACGCAATAATGG | 20,403-20,432 | |
R | GTCACGCAAGACAGTAACATCATGAGGTGG | 24,643-24,614 |