Animal | Viral load (RNA) by RT-qPCR | Proviral load (DNA) by qPCR |
---|
 | Seruma
| Salivab
| Mesenteric lymph nodec
| Bone marrowc
| Spleenc
| Buffy coata
| Salivab
| Mesenteric lymph nodec
| Bone marrowc
| Spleenc
|
#1 | 2.63 × 108
| 1.65 × 105
| 1.31 × 100
| 4.74 × 100
| 1.74 × 100
| 4.26 × 107
| 1.88 × 103
| 2.64 × 10−1
| 5.61 × 10−1
| 3.68 × 10−1
|
#4 | 2.97 × 109
| Ntd
| Nt | Nt | Nt | 3.49 x 107
| Nt | Nt | Nt | Nt |
-
aTotal copy numbers of FeLV RNA or provirus detected per mL of serum or buffy coat, respectively. bTotal copy numbers detected per reaction (5 μL of total nucleic acid – TNA); no absolute quantification was possible since the input saliva volume from the swab sample was not known. cTotal copy numbers detected per reaction in tissue samples, normalized to the copy number of the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene. dNt not tested; samples were not available