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Fig. 2 | Virology Journal

Fig. 2

From: Construction and characterization of an improved DNA-launched infectious clone of duck hepatitis a virus type 1

Fig. 2

Recombinant plasmids digested with appropriate enzymes during construction. Four fragments that amplified with the four pairs primers 3endRibo-F/R, Cloning 2-F/R, DHAV-Seq-6F/9R and 5HeadRibo-F/R were assembled into pIR vector by one multi-step strategy, respectively. The yield recombinant plasmids were digested with appropriate enzymes and verified by electrophoresis in 2% agarose gel. (M) DNA Marker DL2000; (1) The recombinant plasmid that consisted of pIR vector and cloning 1 was digested with BamH I and Xho I; (2) The recombinant plasmid that consisted of pIR vector and cloning 1 and cloning 2 was digested with BamH I and EcoR V; (3) The recombinant plasmid that consisted of pIR vector and cloning 1 to cloning 3 was digested with EcoR V; (4) The complete DNA-launched infectious clone was digested with Asc I and BamH I; (5) The complete DNA-launched infectious clone digested with BamH I; (6) The complete DNA-launched infectious clone digested with Xho I

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