Fig. 7

ICS assays for antigen-specific CD4⁺ and CD8⁺ T-cell secretion of IFN-γ and IL-4 from splenocytes. MARV-specific CD4⁺ and CD8⁺ T cells were measured via ICS assays. Spleens were prepared from 3 mice per group and stained with mouse antibodies against CD4, CD8, IFN-γ and IL-4. CD4⁺ cells secreting IL-4 (a) or IFN-γ (b) and CD8⁺ cells secreting IL-4 (c) or IFN-γ (d) are shown. The data represent the mean ± SD, and statistical significance was analysed using a one-way ANOVA (*p < 0.05, **p < 0.01, and ***p < 0.001)