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Fig. 2 | Virology Journal

Fig. 2

From: Marburg virus-like particles by co-expression of glycoprotein and matrix protein in insect cells induces immune responses in mice

Fig. 2

Western blot analysis of GP and VP40 protein expression in the MARV VLPs. 10 μg of MARV VLPs by co-expression of GP and VP40 were mixed with reducing (with β-mercaptoethanol) protein sample buffer, heated at 95 °C for 5 min, and then subjected to 10% SDS-PAGE with different gels. Two different gels were transferred onto a polyvinylidene fluoride (PVDF) membrane for the Western blot analysis, respectively. a GP was incubated with mouse anti-MARV GP polyclonal antibody (control, lane 1; MARV VLPs, lane 2) and the molecular weight were approximately 150KD. b VP40 proteins was incubated with mouse anti-MARV VP40 polyclonal antibody (MARV VLPs, lane 1; control, lane 2) and the molecular weight were approximately 38KD

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Virology Journal

ISSN: 1743-422X