Fig. 7

Confocal microscopy examination of Viperin with CSFV proteins. a. PK-Vi cells were infected with CSFV (MOI = 0.05) for 48 h, the cells were fixed with 4% paraformaldehyde, permeabilized and immune-stained with anti-E2 antibodies (WH303 mAb), followed by incubation with Cy3 (red)-conjugated goat anti-mouse secondary antibodies. Nuclei were stained with DAPI (Beyotime, Biotech, China) and the cells were examined by confocal microscopy (PE, Ultra View VOX). b. 293 T cells were co-transfected with pcDNA-Vi and pCMV-E2 or pCMV-NS5B and incubated at 37 °C for 48 h. Cells were fixed and immunolabelled for Viperin (rabbit anti-HA Ab) and E2/NS5B (anti-flag mAb) plus Alexa Fluor 488-labeled (green) goat anti-rabbit and Alexa Fluor 555-labeled (red) donkey anti-mouse secondary antibodies. Nuclei were stained with DAPI and examined via confocal microscopy (PE, Ultra View VOX). Scale bar indicated 20 μm