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Fig. 3 | Virology Journal

Fig. 3

From: Sequence-specific detection of different strains of LCMV in a single sample using tentacle probes

Fig. 3

Optimization of annealing temperature for Clone-13 probe and Armstrong probe. a Raw fluorescence measured at different annealing temperatures (45, 50, 55 and 60 °C) of Clone-13 (solid lines) and Armstrong DNA (dashed lines) with the Clone-13 probe. b Normalized Fluorescence to the maximum non-target fluorescence at different annealing temperatures (43, 45, 48 and 50 °C) of Armstrong (color lines) and Clone13 DNA (gray lines) with the Armstrong probe, and c) Raw fluorescence counts at the same annealing temperatures of Armstrong (solid lines) and Clone-13 DNA (dashed lines) with the Armstrong probe

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