Fig. 1
From: Sequence-specific detection of different strains of LCMV in a single sample using tentacle probes
Tentacle probe mechanism of detection of the target. In the annealing step, the capture sequence of the Tentacle Probe hybridizes to the DNA nearby the target sequence to allow proximity of the detection sequence to the target sequence. The detection sequence hybridizes the target sequence enabling the fluorophore to separate from the quencher and read the fluorescence. In the case of a non-target sequence, the detection sequence will not hybridize and consequently the fluorophore will not separate from the quencher. Adapted from [10]