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Fig. 2 | Virology Journal

Fig. 2

From: Identification of a novel linear B-cell epitope using a monoclonal antibody against the carboxy terminus of the canine distemper virus nucleoprotein and sequence analysis of the identified epitope in different CDV isolates

Fig. 2

Characterization of CDV N-reactive mAb. a The immunoreactivity of native CDV N protein to MAb was analyzed with Western blotting. Lane M, PageRuler™ Prestained Protein Ladder (Invitrogen); Lane 1, Cell lysates from uninfected Vero cells; Lane 2, Lysate of Canine distemper virus-infected Vero cells. b Vero cells were infected with CDV3 and used to evaluate mAb reactivity by IFA. c Uninfected Vero cells represent the negative control

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