Table 4 Viral shedding of PPMV-1 from the oral cavity and cloaca of experimentally infected pigeons. Pigeons in groups A1, A2 and A3 were infected with 10⁶ EID₅₀pi/China/SD/2012/132; pigeons in groups B1, B2 and B3 were infected with 10⁶ EID₅₀ pi/China/SH/2013/167. Positive-control group (PC) birds were intramuscularly injected with 10⁶ EID₅₀ F48E8; and negative-control group (NC) birds were inoculated with sterile PBS (pH 7.2). IV, intravenous injection; IM, intramuscular injection; IN, intranasal infection; IO, intraocular infection

IV

IM

IN&IO

IV

IM

IN&IO

IM

IM

5

Oropharyngeal

0/7

0/7

2/7

2/7

1/7

2/7

2/6

0/7

Cloacal

0/7

0/7

0/7

1/7

0/7

0/7

0/6

0/7

7

Oropharyngeal

3/7

1/7

2/7

3/7

6/7

1/7

6/6

0/7

Cloacal

0/7

0/7

0/7

1/7

0/7

1/7

2/6

0/7

10

Oropharyngeal

3/7

4/7

0/7

1/7

3/7

1/7

3/4

0/7

Cloacal

7/7

6/7

1/7

6/7

7/7

1/7

1/4

0/7

14

Oropharyngeal

0/7

0/7

0/7

1/7

0/7

0/7

1/4

0/7

Cloacal

1/7

1/7

0/7

2/7

1/7

1/7

1/4

0/7

21

Oropharyngeal

0/7

0/7

1/7

0/7

0/7

0/7

0/4

0/7

Cloacal

1/7

0/7

0/7

0/7

0/7

0/7

0/4

0/7

28

Oropharyngeal

0/7

0/7

0/7

0/7

0/7

0/7

0/4

0/7

Cloacal

0/7

0/7

0/7

0/7

0/7

0/7

0/4

0/7

35

Oropharyngeal

0/7

0/7

0/7

0/7

0/7

0/7

0/4

0/7

Cloacal

0/7

0/7

0/7

0/7

0/7

0/7

0/4

0/7

42

Oropharyngeal

0/7

0/7

0/7

0/7

0/7

0/7

0/4

0/7

Cloacal

0/7

0/7

0/7

0/7

0/7

0/7

0/4

0/7