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Table 5 Evaluation of different nucleic acid amplification and detection systems in regard to their performance and suitability as POC tests (+++ = good; ++ = medium; + = weak)

From: Development of molecular confirmation tools for swift and easy rabies diagnostics

  R14 RT-qPCR HighSpeed RT-qPCR RT-RPA
Equipment costs + + ++
Equipment size ++ ++ +++
Reagent costs ++ +++ +
Reagent stability + + +++
Rapid assay design ++ ++ +
Reaction speed + ++ +++
Robustness +++ +++ ++
Sensitivity +++ ++ +
Specificity ++ ++ ++