Fig. 4
From: Molecular characterization of the duck enteritis virus US10 protein
The melting curves and standard curves of DEV US10 gene and β-actin by qRT-PCR. a: The melting curve of the DEV US10 gene displayed a single peak at 84.0 °C, while β-actin displayed a single peak at 89.0 °C. b: The standard curves of DEV US10 and β-actin genes were calculated by iCycler IQ 5 software. Each dot represents the result of triplicate amplification of each dilution. The correlation coefficient and the slope of the regression curve were calculated and indicated. The standard curve equation of the DEV US10 gene is Y = −3.320X − 1.340, while that of the β-actin gene is Y = −3.241X − 1.801