Fig. 4
From: New insights into HCV replication in original cells from Aedes mosquitoes
Detection of HCV RNA (+) by one-step Reverse Transcription Polymerase Chain Reaction (RT-PCR). HCV RNA (+) was detected by RT-PCR in non-infected (mock) cells (−) and HCV-infected (+) human HepaRG hepatocytes (a) or insect cells (b, C6/36 Ae. Albopictus and Ktmos1 Ae. Aegypti cells) collected at days 0 (D0), 4 (D4), 21 (D21) and 28 (D28) p.i. (samples). The inoculum HCVsp (LAT isolate, genotype 3) was used as positive control (POS) and load in the last lane (A) and just before the last lane (B). MM, molecular weights markers (in Kb). Agarose gels (1%) were represented and showed products resulting from amplification using 5NC1/2NC2 primers. The specific HCV RNA band corresponded to 243 bp