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Fig. 5 | Virology Journal

Fig. 5

From: ORF1a of highly pathogenic PRRS attenuated vaccine virus plays a key role in neutralizing antibody induction in piglets and virus neutralization in vitro

Fig. 5

Growth kinetics of the rescued viruses in MARC-145 cells. a The growth kinetics of rHuN4-F112, rHuN4-F112-C1a, rHuN4-F112-C1b and rHuN4-F112-C27 in MARC-145 cells. b The growth kinetics of rCH-1R, rCH-1R-H1a, rCH-1R-H1b and rCH-1R-H27 in MARC-145 cells. An asterisk (*) indicates a significant difference in the viral titers between rHuN4-F112 and rHuN4-F112-C1a or between rCH-1R and rCH-1R-H1a (*P < 0.05; **P < 0.01). The pound sign (#) indicates a significant difference between rHuN4-F112 and rHuN4-F112-C1b or between rCH-1R and rCH-1R-H1b (#P < 0.05; ##P < 0.01). Phi (Φ) indicates a significant difference between rHuN4-F112 and rHuN4-F112-C27 or between rCH-1R and rCH-1R-H27 (ΦP < 0.05; ΦΦP < 0.01). The experiments were independently repeated three times and each time point was also independently repeated three times. The results represent the average of the duplicates

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Virology Journal

ISSN: 1743-422X