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Fig. 3 | Virology Journal

Fig. 3

From: The impact of p53 on the early stage replication of retrovirus

Fig. 3

Sequence Analysis of Retrovirus 1-LTR and 2-LTR DNA in Infected Non-Cycling HCT116 p53+/+ and HCT116 p53−/− Cells. a: Design of LTR PCR with primer locations. b. Agarose gel electrophoresis of LTR DNA PCR products. The upper bands were 2-LTR cycle DNA, and the lower bands were 1- LTR cycle DNA. Both 1-LTR and 2-LTR cycle DNA were cloned and then sequenced. c: The overall mutation frequency detected in all clones of 1-LTR and 2-LTR cycle DNA from infected HCT116 p53+/+ and HCT116 p53−/− cells. d. The comparison of number of mutations in the 2-LTR cycle DNA clones between infected non-cycling HCT116 p53+/+ and HCT116 p53−/− cells. e. The comparison of number of mutations in the 1-LTR cycle DNA clones between infected non-cycling HCT116 p53+/+ and HCT116 p53−/− cells. The p value of Fisher’s test is shown above the chart. f. The distribution of mutations in 1-LTR and 2-LTR DNA in infected non-cycling HCT116 p53+/+ and HCT116 p53−/− cells. Mismatches are shown with color bars. Green represents mutant base A; Red represents base mutant T; Orange represents mutant base G; Light blue represents mutant base C; Gray represent gaps and Δ represents insertions

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