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Fig. 9 | Virology Journal

Fig. 9

From: A novel bladder cancer - specific oncolytic adenovirus by CD46 and its effect combined with cisplatin against cancer cells of CAR negative expression

Fig. 9

Cell apoptosis analysis on bladder cancer cells treated with cisplatin and Ad5/F11p. Cell apoptosis were detected by flow cytometry to explore the interaction of Ad5/F11p-PSCAE-UPII-E1A combined with cisplatin. The 5637, EJ, and T24 bladder cancer cells were treated with Ad5/F11p (10 MOI) alone, cisplatin (1 μg/ml) alone, or Ad5/F11p (10 MOI) plus cisplatin (1 μg/ml) respectively for 48 h, incubated with fluorescein isothiocyanate-conjugated annexin V (AV-FITC) and propidium iodide (PI) for 15 min, and then detect cell apoptosis by flow cytometry. The bladder cancer cells were untreated with cisplatin, Ad5/F11p, and Ad5/F11p combined with cisplatin as a control

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Virology Journal

ISSN: 1743-422X