Fig. 4From: Maternal antibodies protect offspring from severe influenza infection and do not lead to detectable interference with subsequent offspring immunizationThe number of maternal immunizations correlate to the level of maternal antibodies and protection against lethal influenza challenge of the offspring. a Design; Adult female mice were vaccinated either 1×, 2× or 3× with Inflexal, or 3× mock immunized with PBS before and/or during mating. Offspring were vaccinated at 3 weeks of age with 0.01 μg Inflexal and subsequently challenged with H1N1 A/Netherlands/602/09 4 weeks post immunization. b The level and protection mediated by maternal antibodies correlate to the number of maternal immunizations; Adult female mice were vaccinated either 1×, 2× or 3× with Inflexal or PBS before and/or during mating. Offspring was immunized with 0.01 μg / HA and subsequently challenged with H1N1 A/Netherlands/602/09 at the age of 7 weeks. One day prior to challenge blood samples were analyzed for homologous recHA A/California/07/09 IgG binding titers. Statistical significance of recHA binding titers and survival percentage compared to the mock immunized group, *p < 0.05, *p < 0.01, ***p < 0.001Back to article page