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Fig. 3 | Virology Journal

Fig. 3

From: Maternal antibodies protect offspring from severe influenza infection and do not lead to detectable interference with subsequent offspring immunization

Fig. 3

Maternal antibodies do not interfere with vaccine induced protection. a At the age of 3 weeks juvenile mice were immunized with trivalent influenza vaccine Inflexal titrated from 0.3 μg HA per strain to 0.003 μg / HA and 4 weeks later challenge with homologous H1N1 A/Netherlands/602/09. Vaccination 0.01 μg H1 HA protects 40% of mice against lethal homologous H1N1 A/Netherlands/602/09 influenza virus challenge. Binding antibody titers 1 day prior to challenge at 7 weeks of age, as measured by recHA A/California/07/09 ELISA assay. Open symbols represents values at LOD. b Adult female mice were vaccinated either 1× or 2× with Inflexal or mock immunized with PBS before and/or during pregnancy. Offspring was either vaccinated with Inflexal or PBS at the age of 3 weeks and subsequently challenged with H1N1 A/Netherlands/602/09 at the age of 7 weeks. An increase in survival was observed only when protective maternal antibodies were present just prior to challenge as detected by homologous recHA A/California/07/09 ELISA, see bottom graphs. *p < 0.05, *** p < 0.001 statistical significance compared to mock immunization (survival %)

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