Fig. 4

Time-dependent inhibition (a, b) and time-of-drug addition approach (c, d) for 6MMPr. CDV-infected cells were treated with 6MMPr (338 μM) and the viral load was measured by qRT-PCR (a) and the TCID₅₀ method (b) at different time points (24, 48 and 72 h) PI. With time-based addition, CDV-infected cells were treated with 6MMPr (338 μM) at 2, 12 and 24 h PI, and inhibition efficiency was quantified with qRT-PCR (C) and TCID₅₀ titration (D). The right vertical axis presents the percentage of virus inhibition highlighted by the markers (♦). The error bars represent standard deviation. Values are the mean ± standard error obtained from three independent experiments (* p < 0.05, ** p < 0.01, *** p < 0.001). BLD, below limit of detection for the TCID₅₀ method (20 TCID₅₀/mL)