Fig. 3

Inhibition effect of CsA on ORFV proliferation in MDBK cells. The MDBK cells infected with ORFV (MOI = 0.1) were exposed to increasing concentrations of CsA (0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2 μg/mL) and maximal ethanol concentration used to solubilize and dilute CsA as vehicle. The anti-ORFV activity of CsA in vitro was detected by MTT assay. Subsequently, the absorbance value at a wavelength of 570 nm was measured for the calculating the inhibitory rate of CsA on ORFV proliferation. The untreated cells were cultured under the same conditions. Data were statistically analyzed by one-way ANOVA followed by Fisher’s LSD test using GraphPad Prism 6 software. Values are the means ± SEM. (*** P < 0.001). The results showed that treatment with 0.2 μg/mL CsA could effectively inhibit the cytotoxicity induced by ORFV