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Table 1 Systemic infection of SYNV antigenomic cDNA derivatives in Nicotiana benthamiana

From: Capped antigenomic RNA transcript facilitates rescue of a plant rhabdovirus

agRNA clone derivativesa Core proteinsb Infectivity (No. of infected/inoculated plants)c
Exp. 1 Exp. 2 Exp. 3 Total (percentage)
35St-SYNV N + P + L 2/50 1/40 2/40 5/130 (3.8%)
35S-HH1-SYNV N + P + L 0/50 0/40 0/40 0/130 (0%)
35St-SYNV NPL + L 8/40 7/35 9/44 24/119 (20.2%)
35S-HH1-SYNV NPL + L 0/40 0/38 0/43 0/121 (0%)
35S-HH3-SYNV NPL + L 0/40 0/35 1/44 1/119 (0.84%)
35St-SYNV-GFPN/P NPL + L 4/24 9/44 7/50 20/91 (21.9%)
35St-SYNV-GFPle/N NPL + L 1/24 2/44 1/50 4/91 (4.4%)
35S-HH3-SYNV NPL + L + N 0/36 0/36 - 0/72 (0%)
  1. aSYNV antigenomic cDNA and its derivatives were cloned into the pCB301 binary vector and positioned downstream of a hammerhead (HH) ribozyme variant or directly under control of a truncated 35S promoter (35St)
  2. bN: pGD-N; P: pGD-P; L: pGD-L; NPL: pGD-NPL containing tandem expression cassettes of N, P and L. pGD vectors expressing BSMV γb, TBSV p19 and TEV P1/HC-Pro RNA suppressors were also included in the mixture
  3. cSystemic infections were verified by visual inspection for systemic symptoms and RT-PCR. The total percentage of systemic infectivity was calculated from independent experiments (Exp.)