Capped antigenomic RNA transcript facilitates rescue of a plant rhabdovirus

Table 1 Systemic infection of SYNV antigenomic cDNA derivatives in Nicotiana benthamiana

agRNA clone derivatives^a	Core proteins^b	Infectivity (No. of infected/inoculated plants)^c
agRNA clone derivatives^a	Core proteins^b	Exp. 1	Exp. 2	Exp. 3	Total (percentage)
35St-SYNV	N + P + L	2/50	1/40	2/40	5/130 (3.8%)
35S-HH1-SYNV	N + P + L	0/50	0/40	0/40	0/130 (0%)
35St-SYNV	NPL + L	8/40	7/35	9/44	24/119 (20.2%)
35S-HH1-SYNV	NPL + L	0/40	0/38	0/43	0/121 (0%)
35S-HH3-SYNV	NPL + L	0/40	0/35	1/44	1/119 (0.84%)
35St-SYNV-GFP_N/P	NPL + L	4/24	9/44	7/50	20/91 (21.9%)
35St-SYNV-GFP_le/N	NPL + L	1/24	2/44	1/50	4/91 (4.4%)
35S-HH3-SYNV	NPL + L + N	0/36	0/36	-	0/72 (0%)

^aSYNV antigenomic cDNA and its derivatives were cloned into the pCB301 binary vector and positioned downstream of a hammerhead (HH) ribozyme variant or directly under control of a truncated 35S promoter (35St)
^bN: pGD-N; P: pGD-P; L: pGD-L; NPL: pGD-NPL containing tandem expression cassettes of N, P and L. pGD vectors expressing BSMV γb, TBSV p19 and TEV P1/HC-Pro RNA suppressors were also included in the mixture
^cSystemic infections were verified by visual inspection for systemic symptoms and RT-PCR. The total percentage of systemic infectivity was calculated from independent experiments (Exp.)

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