Isolation of Tibet orbivirus from Culicoides and associated infections in livestock in Yunnan, China

Table 1 Lengths of dsRNA segments 1–10, encoded putative proteins, 5′and 3′NCRs of DH13C120 virus genome

Segment	Length (bp)	GC%	Protein (aa)	5′NCR (bp)	Terminal sequence (5′–3′)	3′NCR (bp)	Putative function	GenBank Accession no.
S1	3950	41.32	1304	11	GUAAAAUCA---AUACACUUAC	24	VP1: RNA-dependent RNA polymerase	KU754026
S2	2901	40.09	950	13	GUAAAAAUC---UUAAACUUAC	35	VP2: outer capsid protein; controls virus serotype; most variable protein; contains neutralizing epitopes	KU754027
S3	2769	44.42	899	17	GUAAAAUUU---AUACACUUAC	52	VP3(T2): forms sub-core capsid layer	KU754028
S4	1978	42.82	643	8	GUAAAAACA---UUACACUUAC	38	VP4: Cap; protein within the sub-core; shows capping activities and methyltransferase type 1 and type 2 activities	KU754029
S5	1775	43.83	554	31	GUAAAAAAG---UUACACUUAC	79	NS1: TuP; forms tubules of unknown function within the cytoplasm of infected cells	KU754030
S6	1642	43.42	526	31	GUAAAAAGA---UUUCACUUAC	30	VP5: outer capsid protein	KU754031
S7	1165	44.72	349	17	GUAAAAAUU---UUACACUUAC	98	VP7(T13): immunodominant major serogroup specific antigen	KU754032
S8	1142	41.94	359	20	GUAAAAAAU---UUAAACUUAC	42	NS2: single-stranded RNA-binding protein	KU754033
S9	1100	44.73	346	14	GUAAAAAAU---UAAAACUUAC	45	VP6: helicase for unwinding and binding to ssRNA and dsRNA; NTPase	KU754034
S10	832	41.47	234	21	GUAAAAAAG---CCCAACUUAC	106	NS3: involved in cell exit; glycoprotein	KU754035
		42.59	Consensus		GUAAAA^A/_U---------^C/_AACUUAC

ISSN: 1743-422X