Development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus

Table 1 Specificity analysis of MAbs M1 and D2 against begomoviruses and other plant viruses by PTA-ELISA

Antigens	PTA-ELISA (Absorbance 405 nm)
Antigens	MAb M1	MAb D2
Recombinant TYLCTHV-CP protein	1.495	3.274
Partially purified viruses:
TYLCTHV from tomato	1.800	2.399
ToLCNDV from cucumber	0.110	2.648
SLCCNV from ivy gourd	0.107	0.711
SLCCNV from pumpkin	0.081	2.049
SLCCNV from wax gourd	0.086	1.788
ToLCNDV from loofah	0.101	2.944
Coating buffer	0.106	0.096
Sap extracts:
TYLCTHV-infected tomato	1.262	0.928
ToLCNDV-infected cucumber	0.091	2.442
SLCCNV-infected pumpkin	0.126	1.783
SLCCNV-infected wax gourd	0.121	1.424
ToLCNDV-infected loofah	0.111	0.638
TbLCYnV-infected tomato	0.375	0.534
BYVMV-infected okra	0.110	0.104
PepYLCIV-infected pepper	0.120	0.105
TYLCKaV-infected eggplant	0.130	0.100
TMV-infected leaves	0.118	0.173
CMV-infected leaves	0.124	0.189
PRSV-infected pumpkin	0.114	0.184
WMV-2-infected leaves	0.115	0.171
WSMoV-infected Physalis minima	0.124	0.184
CaCV-infected Physalis minima	0.106	0.153
MYSV-infected Physalis minima	0.111	0.167
TNRV-infected Physalis minima	0.120	0.183
CGMMV-infected cucumber	0.122	0.191
CABYV-infected bitter gourd	0.113	0.157
Healthy tomato	0.093	0.111
Healthy cucumber	0.116	0.171
Healthy pumpkin	0.108	0.160
Healthy wax gourd	0.083	0.103
Healthy loofah	0.121	0.161
Healthy bitter gourd	0.112	0.175

ISSN: 1743-422X