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Table 1 Specificity analysis of MAbs M1 and D2 against begomoviruses and other plant viruses by PTA-ELISA

From: Development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus

Antigens PTA-ELISA (Absorbance 405 nm)
MAb M1 MAb D2
Recombinant TYLCTHV-CP protein 1.495 3.274
Partially purified viruses:
 TYLCTHV from tomato 1.800 2.399
 ToLCNDV from cucumber 0.110 2.648
 SLCCNV from ivy gourd 0.107 0.711
 SLCCNV from pumpkin 0.081 2.049
 SLCCNV from wax gourd 0.086 1.788
 ToLCNDV from loofah 0.101 2.944
 Coating buffer 0.106 0.096
Sap extracts:
 TYLCTHV-infected tomato 1.262 0.928
 ToLCNDV-infected cucumber 0.091 2.442
 SLCCNV-infected pumpkin 0.126 1.783
 SLCCNV-infected wax gourd 0.121 1.424
 ToLCNDV-infected loofah 0.111 0.638
 TbLCYnV-infected tomato 0.375 0.534
 BYVMV-infected okra 0.110 0.104
 PepYLCIV-infected pepper 0.120 0.105
 TYLCKaV-infected eggplant 0.130 0.100
 TMV-infected leaves 0.118 0.173
 CMV-infected leaves 0.124 0.189
 PRSV-infected pumpkin 0.114 0.184
 WMV-2-infected leaves 0.115 0.171
 WSMoV-infected Physalis minima 0.124 0.184
 CaCV-infected Physalis minima 0.106 0.153
 MYSV-infected Physalis minima 0.111 0.167
 TNRV-infected Physalis minima 0.120 0.183
 CGMMV-infected cucumber 0.122 0.191
 CABYV-infected bitter gourd 0.113 0.157
 Healthy tomato 0.093 0.111
 Healthy cucumber 0.116 0.171
 Healthy pumpkin 0.108 0.160
 Healthy wax gourd 0.083 0.103
 Healthy loofah 0.121 0.161
 Healthy bitter gourd 0.112 0.175
  1. Cut-off value = two times the absorbance (OD405) of the healthy control
  2. Bold letter indicates ODs higher than the associated cut-off value