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Fig. 2 | Virology Journal

Fig. 2

From: Specific and quantitative detection of Human polyomaviruses BKPyV and JCPyV in the healthy Pakistani population

Fig. 2

Amplification and standard curves for JCPyV dilutions. A standard curve was generated using duplicate ten-fold serial dilutions of purified LT-JCPyV-pcDNA3 template. The linear range was set to lie between 106 to 100 copies/reaction. The assay efficiency was checked by (a) amplification plots of JCPyV standards dilution (b) Melt curves analysis for JCPyV target gene with a peak at 77 °C (c) standard curve analysis for JCPyV dilutions. The assay efficiency was calculated 109% with R2 = 0.99. d The amplified product of each dilution was analyzed on agarose gel. Lane 1: molecular mass marker; Lane 2–8: dilution (copy number 106 to 100); Lane 9: negative control

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Virology Journal

ISSN: 1743-422X