Table 1 Primers used in this paper
NO. | Primers | Sequence (5’-3’) | Product |
|---|---|---|---|
1 | TKA-HOMO-for | gaattcatgcttgccatcataaccgtattctc | TK left homology arm |
TKA-HOMO-rev | tctagaataacttcgtataatgtatgctatacgaagttatcacctcgagcttttctttcctgtg | ||
2 | TKB-HOMO-for | gcatgcacatagcaacaactgacgcaaaagc | TK right homology arm |
TKB-HOMO-rev | aagctttcccagaaagctcgcctaggtcctc | ||
3 | EGFP-for | tctagatagttattaatagtaatcaattacg | EGFP |
EGFP-rev | gtcgacatgcagtgaaaaaaatgct | ||
4 | sopB-for | attcgttaattgcgcgcgtagg | sopB |
sopB-rev | gaatattcaggccagttatgct | ||
5 | repA-for | catggcggaaacagcggttatc | repA |
repA-rev | atgtatgagaggcgcattggag | ||
6 | TK-for | cgcggatcccactgaatgtcactgc | TK |
TK-rev | cccaagctttcaattaattgtcatctcggt | ||
7 | ΔUL55-KanR-for | gaaaggcggttggaataagaggaacgaggcggtagacgtgaccgacaacagtgtaggctggagctgcttc | KanR gene flanked by homology arms of UL55 |
ΔUL55-KanR-rev | tttcttatggttttaataaaacgctttattacattgtagtgtaacaagaccatatgaatatcctccttag | ||
8 | ΔUL55/ΔUL55R-for | tgcaaattagtgggaggtacg | ΔUL55/ΔUL55R identification product |
ΔUL55/ΔUL55R-rev | cccaaataccctgttagtagctt | ||
9 | ΔUL55R-UL55-for | atggccgacgcgaaggcggt | UL55 fragment with left homology arm of UL55 |
ΔUL55R-UL55-rev | gaagcagctccagcctacactcatacattagctttgtg | ||
10 | ΔUL55R-KanR-for | cacaaagctaatgtatgagtgtaggctggagctgcttc | UL55 fragment with right homology arm of UL55 |
ΔUL55R-KanR-rev | tttcttatggttttaataaaacgctttattacattgtagtgtaacaagaccatatgaatatcctccttag |