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Fig. 1 | Virology Journal

Fig. 1

From: Glucose-regulated protein 78 demonstrates antiviral effects but is more suitable for hepatocellular carcinoma prevention in hepatitis B

Fig. 1

GRP78 mainly inhibited HBV replication and reduced virion production. HepG2.2.15 cells were regularly cultivated and transfected with pGRP78 (GRP78-expressing recombinant plasmid generated from pCMV6-XL5) or siRNA targeted GRP78 (siGRP78) or their controls, pCMV6-XL5 (pVector) and siRNA control (siControl). a GRP78, detected as two bands, perhaps due to splice variation, was successfully up-regulated or down-regulated 72 h after transfections. b GRP78 overexpression decreased and down-regulation increased the supernatant level of core-associated HBV DNA 72 h after transfections. Results are expressed as n-fold times the supernatant HBV DNA level of test groups over those of controls. **P < 0.01. c The similar effect of GRP78 on the intracellular core-associated HBV DNA was observed. Relaxed circular (RC) HBV DNA was the HBV genome, and single stranded (SS) HBV DNA, other bands and the smear were the replication intermediates (RI) of HBV. d GRP78 showed an opposite effect on the supernatant level of core-associated HBV DNA 24 h after transfections. Results are expressed as n-fold times the HBV DNA level of test groups over those of controls. *P < 0.05, **P < 0.01

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Virology Journal

ISSN: 1743-422X