Table 3 Oligonucleotide primers for detection of AIV, NDV, IBV, and IBDV
From: New oligonucleotide microarray for rapid diagnosis of avian viral diseases
Name | Primer sequence (5` → 3`) | Position in genome | PCR product size, bp |
|---|---|---|---|
IBDV-VP2 | (F) GAGCTGATCCCAAATCCTGAA (R) GCGTCTTCCACTGTCGTAATAA | 1244–1264 1836–1815 | 593 |
IBV-S1 | (F) TATGGCAGAACTGGCCAAGG (R) AAGGTGCCACAAACTGTTCC | 21,622–21,641 22,042–22,023 | 421 |
NDV-NP | (F) ATGACATTGCTAGGCGACAG (R) GAATTGTGTCTCTCCGTCCC | 1051–1070 1461–1442 | 411 |
AIV Flu-NP | (F) GGAACCACCAACCAACAGA (R) TCCTCTGCATTGTCTCCGAA | 1180–1198 1484–1465 | 305 |
AIV Flu-M | (F) TCTCATAGGCAAATGGTGGC (R) AGACTCAGGTACTCCTTCCG | 506–525 926–907 | 421 |