Fig. 2
From: Study of SV40 large T antigen nucleotide specificity for DNA unwinding
Helicase assay results of LT nucleotide pocket mutants. a, b The helicase activity assay results for the polar/charged residue mutants a and hydrophobic residue mutants b are shown in gel analysis data and quantitative bar charts. The native gels shown are a single representation from a set of three individual experiments. Each lane (A: ATP, T: TTP, U: UTP, C: CTP, and G: GTP) contains 0.5 mM of each respective NTP, 100 fmol of a 3’-6FAM labeled dsDNA substrate, 90 nM of each respective LT mutant. The ─NTP controls undergo the same reaction without NTP; the ssDNA lane contains the non-annealed labeled ssDNA as the unwound ssDNA control. The lower bands indicate the unwound labeled ssDNA strand. The bar graphs represent the average of three individual experiments in the percentage of strand displacement. Changes were calculated via unpaired t-tests and Bonferroni corrected (n = 16) with a p-value of 0.0031 as threshold for statistical significance, noted with ***