Development of a simplified RT-PCR without RNA isolation for rapid detection of RNA viruses in a single small brown planthopper (Laodelphax striatellus Fallén)

Table 1 Primers used for RT-PCR or qRT-PCR amplification of RSV, RBSDV and HiPV

Name	Sequences (5′→3′)	Accession number	Amplicon length (bp)
RSV CP-F1	ATGGGTACCAACAAGCCAGC	EF198700	936
RSV CP-R1	CTAGTCATCTGCACCTTCTG
RBSDV P10-F	ATGGCTGACATAAGACTCGA	NC_003733	1677
RBSDV P10-R	TCATCTTGTCACTTTGTTTA
HiPV-F	CTGGACAACATGATATTAGA	AB183472	678
HiPV-R	CTATTTCCCAGTTCCAAG
RSV CP-F2	GCCACTCTAGCTGATTTGCA	EF198700	167
RSV CP-R2	GTGTCACCACCTTTGTCCTT

ISSN: 1743-422X