Table 1 E6/E7 mRNA expression of each genotype and oncogenes positivity rate according to three RNA extraction methods
From: RNA extraction method is crucial for human papillomavirus E6/E7 oncogenes detection
Genotype (n° of samples) | E6/E7mRNA expression (n°, %) | ||
|---|---|---|---|
Nuclisens manual extraction kit (bioMérieux) | High Pure Viral RNA Kit (Roche) | RNeasy Plus Mini kit (Qiagen) | |
HPV 16 (32) | 22/68.75 | 6/18.75 | 2/6.25 |
HPV 18 (6) | 5/83.33 | 3/50.00 | 0/0.00 |
HPV 31 (5) | 2/40.00 | 1/20.00 | 1/20.00 |
HPV 33 (3) | 3/100.00 | 2/66.67 | 0/0.00 |
HPV 45 (7) | 2/28.57 | 2/28.57 | 0/0.00 |
E6/E7mRNA positivity rate (%) | 62 | 24 | 6 |