Table 2 Primers used for in vitro mutagenesis, PCR, qPCR and RT-qPCR in this study
Primer number | Sequence | Target Gene (construct) | Purpose | Reference |
|---|---|---|---|---|
823 | CTTCCCAACTCTATGGGTGATGGCTAGCCTAGGAGTAACATCACTGACACATCG | EACMV-K201 (DNA-A) | Mutagenesis primer (introduces Avr II and Nhe I) | This study |
824 | CGATGTGTCAGTGATGTTACTCCTAGGCTAGCCATCACCCATAGAGTTGGGAAG | |||
825 | ATAGATGCGTATTTTAAGCGTCGCACCTGCAGGATTTGAGGCATGTGTACATGC | EACMV-K201 (DNA-A) | Mutagenesis primer (introduces Sbf I) | This study |
826 | GCATGTACACATGCCTCAAATCCTGCAGGTGCGACGCTTAAAATACGCATCTAT | |||
857 | GCTAGCCCAACACTTGTCACTACTTTC | erGFP | GFP-VIGS | This study |
858 | CCTGCAGGGAAAGGGCAGATTGTGTGGA | |||
907 | GCTAGCGCTGAGTCATATCAGAAGGC | MeSPY1/Manes.09G052300.1 | MeSPY1-VIGS | This study |
908 | CCTGCAGGATACCTTGATTGATGTCTCC | |||
1260 | CCTGCAGGTAGACGTGAAACCAGATATTGTGC | MePSY2/Manes.01G124200.1 | MePSY2-VIGS | This study |
1261 | GCTAGCAACACTCATTAATCCAACCGTCCC | |||
1066 | CAAAGGTTAAATTGGAGGGAGACATC | MeSPY1/Manes.09G052300.1 | RT-qPCR | This study |
1067 | GCATTTCACCATATGCAACACCAAGA | |||
833 | TTGCAGAGGAAGATAGTGGGAATG | EACMV-K201 (DNA-A) | Probe for Southern blot | Beyene, et al. [15] |
834 | GAACGTGATGGGTTCCGCTG | |||
837 | TGCAAGGCTCACACTTTCATC | MePP2A/Manes.09G039900.1 | Reference gene for qPCR | Moreno, et al. [27] |
838 | CTGAGCGTAAAGCAGGGAAG | |||
1046 | GGTCTTCCCTGTACGACTATC | EACMV-K201 (DNA-A) | qPCR (virus load) | Beyene, et al. [15] |
1047 | GGAACTTGAAGTCTGGGTTTCC |