Fig. 1

East African Cassava mosaic virus (EACMV-K201) infectious clone-based virus-induced gene silencing (VIGS) vector. a Coat protein (CP) nucleotide sequence of EACMV-K201 DNA-A showing position and mutated nucleotides that introduced Avr II and Nhe I near 5′-end and Sbf I near 3′-end. Nucleotide positions were counted from the start codon “ATG” of the CP. b Infectivity of the GFP-VIGS and MePSY2-VIGS targeting GFP and cassava phytoene synthase coding sequences, respectively, in wild-type cassava cultivar TME 7S. GFP-VIGS has no known target in the challenged cassava plant, and these plants display typical mosaic symptoms characteristic of CMD on leaves. MePSY2-VIGS-challenged plants show chlorosis on leaves. Pictures were collected at 2 and 12 weeks post inoculation (WPI)