Fig. 8
From: A mutant Tat protein inhibits infection of human cells by strains from diverse HIV-1 subtypes
NB-ZSG or ZSG expression does not affect CD4+ T cell viability, proliferation or induce apoptosis. a An MTS assay was used to measure the viability of CD4+ T cells expressing NB-ZSG1 or ZSG1 or NT. b The proliferation of CD4+ T cells expressing NB-ZSG1 or ZSG1 or NT was measured using VPD450 dye assay. c An apoptosis assay using PE Annexin V was used to monitor levels of apoptosis in CD4+ T cells expressing NB-ZSG1 or ZSG1 or NT. All assays were performed in triplicate and mean values and standard deviations are shown