Fig. 5

Co-localization between M1 and NEP, Hsc70 and NEP, Hsc70 and M1 in Hela cells. Hela cells were transfected with pEGFP-M1, pDsRed2-NEP or pEYFP-Hsc70 (a), plasmids pEGFP-M1 and pDsRed2-NEP, pEYFP-Hsc70 and pDsRed2-NEP, or pEYFP-Hsc70 and pEGFP-M1 (b). At 24 h post-transfection, cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton X-100 and incubated DAPI. Cells were imaged on a LSM510 Meta confocal laser-scanning microscope