Table 1 Primers for construction of enkaryotic expression vectors
Primer | Sequence (5′-3′) | Use |
|---|---|---|
HA-NF45-F | CCGGAATTCGGATGAGGGGTGACAGAG | Construction of pCMV-HA-NF45 |
HA-NF45-R | CCGCTCGAG TCACTCCTGAGTTTCCATG | |
HA-PCNA-F | CGCGTCGACCATGTTCGAGGCGCGCC | Construction of pCMV-HA-PCNA |
HA-PCNA-R | CCGCTCGAGCTAAGACCCTTCTTCATCTTCG | |
HA-M-F | CCGGAATTCGGATGGGGTCGTCTCTAGACG | Construction of pCMV-HA-M |
HA-M-R | CCGCTCGAG TTATTTGGCATATTTAACAAGG | |
Flag-NF45-F | CCGGAATTCGGATGAGGGGTGACAGAG | Construction of pCAGGS-Flag-NF45 |
Flag-NF45-R | CCGCTCGAGCACTCCTGAGTTTCCATG | |
Flag-PCNA-F | GCCGAGCTCAATGTTCGAGGCGCG | Construction of pCAGGS-Flag-PCNA |
Flag-PCNA-R | CCGCTCGAGTAAGACCCTTCTTCATCTTCG |