Fig. 2

Reconstituted RNP Expression System. 293 T cells are transfected with plasmids to express mRNAs encoding the viral RdRP and NP or NP substitution mutants, and a specific vRNA or cRNA template. Cellular RNA polymerase II drives expression of viral mRNAs from pcDNA3 plasmid backbone while cellular RNA polymerase I drives expression of the cRNA or vRNA template from pHH21 plasmid backbone. vRNP function is assessed by gene expression from vRNA or cRNA templates