Fig. 7

Granzyme B is produced in the TG of C57BL/6 mice by CD8⁺ T/NK after infection. a The GRZ-b mRNA level was measured in TG and spleen homogenates from C57BL/6 (WT) and TLR2/9^−/− (KO) mice on the 5^th day post infection (10⁶ p.f.u. of HSV-1) by qPCR. Data are representative of two independent experiments with similar results (n = 9–19 mice/group). The frequencies of GRZ-b-producing cells within the population of live leukocytes were analyzed by flow cytometry in the TG and the spleen b–f (6 animals per group). b GRZ-b⁺ NK T cells (CD45⁺CD3⁺NK1.1⁺); c GRZ-b⁺ CD4⁺ T cells (CD45⁺CD3⁺CD4⁺); d NK cells (CD45⁺NK1.1⁺); e GRZ-b⁺ CD8⁺ T cells (CD45⁺CD3⁺CD8⁺) and f GRZ-b⁺ live leucocytes (CD45⁺GRZb⁺). A minimum of 100,000 events was acquired for analysis. White bars: mock-infected groups. Black bars: infected groups. ND: not detected. Bars show the means ± SEM. * p < 0.05; ** p < 0.01 and *** p < 0.001