Fig. 2

DCs are the major producers of IL-1β in the TG of C57BL/6 mice after infection. a Peritoneal macrophages derived from C57BL/6 (WT) and TLR2/9^−/− (KO) mice were infected with HSV-1 (m.o.i. of 1, 5 wells/group), and the levels of IL-1β mRNA were determined in the supernatants at different times after infection (as indicated in the figure) by qPCR. The results use arbitrary units for the ratio of target gene mRNA to endogenous control HPRT mRNA. Data are representative of two independent experiments with similar results. For myeloid cell and DC frequency analyses, groups of six mice were infected with 10⁶ p.f.u. HSV-1 via the intranasal route and, on the 5^th day post infection, mice were euthanized, and TG and spleen cell suspensions prepared for flow cytometry evaluation. The frequencies of myeloid (CD45⁺CD3⁻CD11b⁺) and dendritic (CD45⁺MHC class II ^high+CD11c⁺) cells producing IL-1β in the TG (b) and spleen (c) are shown. The frequency of each cell was calculated within the population of live leukocytes. A minimum of 100,000 events was acquired for analysis. ND: not detected. Bars show the means ± SEM. * p < 0.05 and ** p < 0.01