Coxsackievirus B4 vertical transmission in a murine model

Table 1 RT-PCR results for viral RNA detection in several tissues following CV-B4 E2 inoculation

	Sampling		Day 17G			Day 0			Day 5
Inoculation at day 10G	Dams	Uterus	+*									Litter 1
	Dams	Uterus	+									Litter 2
	Offspring	Amniotic fluid	+*									Litter 1
		Amniotic fluid	-									Litter 2
		Placenta	+*	-	-							Litter 1
		Placenta	+	+	-							Litter 2
		Pancreas	+	+*	+	+	+*	+*	+	+	+*	Litter 1
		Pancreas	+	+	+	+*	+*	+	+	+	+	Litter 2
		Heart	-	+	+	+	+	+*	+	+	+	Litter 1
		Heart	+	-	-	+*	+	+	+	+	-	Litter 2
Inoculation at day 17G	Offspring	Pancreas				+	+*	+	+	+	+	Litter 1
		Pancreas				+	+	-	+	+	+	Litter 2
		Heart				+*	+	-	+	+	+*	Litter 1
		Heart				+*	+*	-	+	+	+	Litter 2
			pup 1	pup 2	pup 3	pup 1	pup 2	pup 3	pup 1	pup 2	pup 3

Uteri from two pregnant dams inoculated at day 10G, together with amniotic fluid (pool) and placentas of three offspring of each dam, were sampled at day 17G. Pancreases and hearts from six offspring (each three born to one different dam) were sampled at each of days 17G, and 0 and 5 post-partum when dams were inoculated at day 10G, and at days 0 and 5 post-partum when dams were inoculated at day 17G. Results are summarized as positive (+) or negative (-). * Result obtained by sn-RT-PCR

ISSN: 1743-422X