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Table 1 RT-PCR results for viral RNA detection in several tissues following CV-B4 E2 inoculation

From: Coxsackievirus B4 vertical transmission in a murine model

  Sampling Day 17G Day 0 Day 5  
Inoculation at day 10G Dams Uterus +*   Litter 1
+ Litter 2
Offspring Amniotic fluid +* Litter 1
- Litter 2
Placenta +* - - Litter 1
+ + - Litter 2
Pancreas + +* + + +* +* + + +* Litter 1
+ + + +* +* + + + + Litter 2
Heart - + + + + +* + + + Litter 1
+ - - +* + + + + - Litter 2
Inoculation at day 17G Offspring Pancreas   + +* + + + + Litter 1
+ + - + + + Litter 2
Heart +* + - + + +* Litter 1
+* +* - + + + Litter 2
  pup 1 pup 2 pup 3 pup 1 pup 2 pup 3 pup 1 pup 2 pup 3  
  1. Uteri from two pregnant dams inoculated at day 10G, together with amniotic fluid (pool) and placentas of three offspring of each dam, were sampled at day 17G. Pancreases and hearts from six offspring (each three born to one different dam) were sampled at each of days 17G, and 0 and 5 post-partum when dams were inoculated at day 10G, and at days 0 and 5 post-partum when dams were inoculated at day 17G. Results are summarized as positive (+) or negative (-). * Result obtained by sn-RT-PCR
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