Fig. 4

Profiles of lipid metabolism associated gene transcripts in response to infection of HEK293T/17 cells with DENV 4. HEK293T/17 cells were either infected with a laboratory adapted DENV-4LAB (grey bars) or a low passage DENV-4DHF (black bars) as previously described [27] or mock infected (white bars) and the expression of fatty acid synthase (FASN), acetyl CoA carboxylase alpha (ACC1), adipose triglyceride lipase (ATGL), peroxisome proliferator activated receptor (PPARα), diglyceride acyltransferase (DGAT), fatty acyl CoA oxidase (AOX), carnitine palmitoyltransferase I (CPT1), medium-chain acyl-CoA dehydrogenase (MCAD), sterol regulatory element-binding protein-1c (SREBP1c) and stearoyl-CoA desaturase (SCD1) genes analyzed by real time PCR on days 1–3 p.i. Experiment was undertaken independently in triplicate. Bars show mean +/−SD (*; p value <0.05)