Fig. 10

Double staining for FASN and NS3. HEK293T/17 cells were pre-treated with 10 μM orlistat or not treated before mock infection or infection with DENV 2. At (a) 24 or (b) 36 h.p.i cells were fixed and stained to show the FASN (green), DENV 2 NS3 protein (red) and nuclei (blue). Cells were examined under an Olympus FluoView 1000 confocal microscope with 60× magnification. Representative, non-contrast adjusted merged images are shown