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Table 2 q-PCR results for sensitivity of reference

From: Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus

HB29 strain

Ct value

SinoMD kit

DaAn kit

Exp 1

Exp 2

Exp 3

Exp 4

Exp 1

Exp 2

Exp 3

Exp 4

S1 (105 U/mL)

30.03

27.35

29.40

30.23

21.42

22.48

26.37

24.11

S2 (104 U/mL)

32.21

33.16

32.74

33.27

26.05

24.84

28.86

26.69

S3 (103 U/mL)

37.07

34.04

35.60

36.19

28.28

28.32

30.75

28.13

S4 (102 U/mL)

37.36

32.20

29.46

31.79

31.08

S5 (10 U/mL)

32.37

32.06

31.17

Positive

17.59

16.75

17.84

19.07

19.77

23.49

20.69

20.00

Negative

  1. Exp experiment