TLR2 2258 G>A single nucleotide polymorphism and the risk of congenital infection with human cytomegalovirus

Table 1 Primers, annealing temperatures and amplicons used in PCR assays for SNPs in the TLR2 gene

Gene	GenBank accession no.^a	SNP^b name	Primer sequences (5′-3′)		Annealing temperature [°C]	Amplicon length (bps)^c
TLR2	NC_000004.12	1350 T>C	External	For: AATTCAGCCTGTGAGGATGC	52	361
		(rs3804100)	External	Rev: GTAAGAGGGAGGCATCTGGTA	52	361
		(Ser450)	Internal	For: TCATTTGGCATCATTGGAAA	55	248
		(S450)	Internal	Rev: GAGTTGCGGCAAATTCAAAG	55	248
		2029 C>T; 2258 G>A	External	For: CGGAATGTCACAGGACAGC	52	605
		(rs121917864; rs5743708)	External	Rev: GGACTTTATCGCAGCTCTCAG	52	605
		(Arg677Trp; Arg753Gln)	Internal	For: GCCTACTGGGTGGAGAACCT	59	340
		(R677W; R753Q)	Internal	Rev: GGCCACTCCAGGTAGGTCTT	59	340

ISSN: 1743-422X