Fig. 2
From: Acute flaccid myelitis associated with enterovirus-D68 infection in an otherwise healthy child
Phylogenetic tree: Sequence analysis showed that the strains obtained in 2016 (in red) are closely related to sequences of the recently described subclade B3, represented by four sequences from China (in green, 2014). The sequence from our Italian acute flaccid myelitis case (in light blue) is closely related to Dutch acute flaccid myelitis EV-D68 cases from 2016. Other 2014 cases are in dark blue. The nucleotide divergence was 2.1% within B3, 5.5% between B1 and B3, and 7.3% between B2 and B3. As outlier, the Fermon strain from 1962 was used. Analysis was performed using Bionumerics Software (Biomerieux, France) with sequencing partial VP1