Fig. 5

Cytokine and TLR profiles in the bursa of SPF chickens inoculated with DT40 cell-derived vvIBDV. a Quantification of viral loads in the bursa of vvIBDV-infected chickens by real-time RT-PCR. Total RNA isolated from the IBDV-infected bursa at the indicated time points was analyzed by absolute real-time RT-PCR analysis of VP2 RNA. The values are the means of the results for the three chickens in each group; error bars show the standard deviations. Expression levels of inflammatory cytokines b and TLRs (c) in the bursa of vvIBDV-infected chickens are shown. Total RNA isolated from bursa samples of infected or sham-infected chickens (n = 3 per group) at all time points after vvIBDV infection were analyzed by real-time RT-PCR analysis to detect inflammatory cytokine and TLR transcripts. The data were normalized to the amount of GAPDH mRNA. The data are expressed as percentages of the normalized value for the vvIBDV-infected bursa versus the sham-infected bursa; the values are the means ± SD of values from three chickens. The statistical analysis was performed by comparing data from sham-infected chickens. *P < 0.05