Fig. 2

Levels of anti-VHSV antibodies in immunized muskellunge following VHSV challenge. Levels of circulating anti-VHSV antibodies of 15 muskellunge following vaccinated with 10 μg of the pVHSivb-G and VHSV immersion challenged [(2 × 103 pfu mL−1) for 60 min] indicated by indirect ELISA optical density (OD490) values. Predicted relationships from 0.50, 0.75, and 0.90 quantile regression models fit to the OD values as a function of time since vaccination. The dashed lines represent ±1 SE of the predicted relationships. The cut off value was estimated to be 0.163, above which a sample was considered positive