Table 1 Primers used in cloning and mutagenesis
From: Construction of a molecular clone of ovine enzootic nasal tumor virus
Primer Name | Sequence 5’ to 3’ |
|---|---|
E5F | AGCAAGGATCAGCCATTCT |
E5R | GAACACAGATAAAGGGAGGC |
E3F | TAACACCCAACTAGTAAAGCTG |
E3R | GGCTGATACCTTGCTTTATTG |
CMVF | GCTCTAGACGTTACATAACTTACGGTAAATGGCC |
RCMVR | AGAATGGCTGATACCTTGCCGGAGGCTGGATCGGTC |
EGagR | TCCCCTACCTTTTTCCATGTT |
JEenvR | ATTTCTCTATCCCATCCTAAGG |
EU5F | CCCATCTTTTGCCTCTCTTAT |
EenvR | CTCCGTTTTGTATCCGTTGC |
JU5F | CCCCATCTTTTGTCTCTCTCTT |
JenvR | CCCATTTTGTACCCGCTGT |
EproS199P F | GGCCTACTACATGGCCAAAACAGACGGCTATTTCC |
EproS199P R | AAATAGCCGTCTGTTTTGGCCATGTAGTAGGCCAAT |
EproT202M F | ACATGGCCAAAACAGATGGCTATTTCCACTCT |
EproT202M R | GAGTGGAAATAGCCATCTGTTTTGGCCATGTA |
EproT289I F | AAACAACATCAAGGCATCATTTTGCCCCTTGAT |
EproT289I R | ATCAAGGGGCAAAATGATGCCTTGATGTTGTTT |