Fig. 2
From: Construction of a molecular clone of ovine enzootic nasal tumor virus
Dose dependent rescue of ENTV-1 polyprotein processing defect by complementation with JSRV Gag-Pro-Pol. a Capsid protein immunoblot analysis of virus particles obtained from HEK 293T cells co-transfected with the ENTV-1 molecular clone and the JSRV Gag-Pro-Pol expression vector (ENTV-1/J-GP) showing protease processing of the Gag polyprotein (lane 4). Virus derived from transfection of the JSRV (lane 2) and ENTV (lanes 3) molecular clones. The dosage dependence of polyprotein processing was evaluated by co-transfection of consistent amounts of JSRV Gag-Pro-Pol (0.5ug) with increasing amounts of ENTV-1 (1 to 10 μg) (b) as well as consistent amounts of ENTV-1 (5 μg) and increasing amounts of JSRV Gag-Pro-Pol (0.5 to 5 μg) (c). M indicates the lane containing the molecular weight marker. The black arrows indicate unprocessed Gag at 78 kDa and the red arrows indicate processed Capsid at 26 kDa